Location
University of Nevada Las Vegas, Student Union Ball Room
Start Date
6-8-2009 9:30 AM
End Date
6-8-2009 12:00 PM
Description
The relA gene in Bacillus subtilis controls a variety of factors during the stringent response which is a response to starvation of amino acids. The stringent response inhibits DNA synthesis and transcription of genes of tRNA, rRNA, and ribosomal proteins and promotes synthesis of the required amino acids. The objective of my project is to determine if a strain of B. subtilis that has a knockout mutation for the relA gene will accumulate a higher number of mutations that confer resistance to antibiotics that inhibit translation. It is proposed that because the relA gene inhibits transcription of ribosomal proteins, a strain lacking this gene will transcribe more rRNA and ribosomal proteins and promote the generation of mutations that target the translation process.
Keywords
Antibiotic resistance; Amino acids; Bacillus subtilis; Ribosomal proteins
Disciplines
Medical Microbiology | Medical Pharmacology
Language
English
Mutations to antibiotic resistance during stringent response in B. subtilis
University of Nevada Las Vegas, Student Union Ball Room
The relA gene in Bacillus subtilis controls a variety of factors during the stringent response which is a response to starvation of amino acids. The stringent response inhibits DNA synthesis and transcription of genes of tRNA, rRNA, and ribosomal proteins and promotes synthesis of the required amino acids. The objective of my project is to determine if a strain of B. subtilis that has a knockout mutation for the relA gene will accumulate a higher number of mutations that confer resistance to antibiotics that inhibit translation. It is proposed that because the relA gene inhibits transcription of ribosomal proteins, a strain lacking this gene will transcribe more rRNA and ribosomal proteins and promote the generation of mutations that target the translation process.
Comments
Abstract & poster