Document Type

Poster

Publication Date

2013

Publisher

University of Nevada, Las Vegas; Center for Academic Enrichment and Outreach

Abstract

‘Aigarchaeota’ is a candidate phylum of Archaea known only by 16S rRNA gene fragments from cultiva­tion-independent microbial surveys and a single composite genome from Candidatus ‘Caldiarchaeum sub­terraneum’, an inhabitant of a subterranean gold mine in Japan. Gene sequences associated with ‘Aigarchaeo­ta’ have been found in a variety of geothermal habits, however a comprehensive analysis of the phylogeny and distribution of ‘Aigarchaeota’ has not yet been done. Public databases were mined for 16S rRNA gene sequences related to known ‘Aigarchaeota’ and a combination of approaches were used to rigorously define the phylogenetic boundaries of the phylum, investigate its distribution, and design potential ‘Aigarchaeota’- specific primers. Approximately 300 16S rRNA genes and gene fragments affiliated with ‘Aigarchaeota’ were identified, phylogenic analyses suggested that ‘Aigarchaeota’ belonged to at least three family- to order-level groups and at least 13 genus-level groups, and supported the proposed relationship between ‘Aigarchaeota’, Thaumarchaeota, Crenarchaeota, and Korarchaeota in the so-called ‘TACK’ superphylum. A global distri­bution was suggested with genus-level group habitat differentiation. Some groups were predominantly ter­restrial (1A, 2B, 3, 4 & 5) while other groups were mostly found in Marine habitats (6-8). Fifteen strong ‘Ai­garchaeota’-specific primer candidates for the polymerase chain reaction (PCR) amplification of 16S rRNA genes were designed for further analyses.

Keywords

Archaebacteria; Genes; Phylogeny

Disciplines

Genetics | Genetics and Genomics | Life Sciences

Language

English


Included in

Genetics Commons

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