Award Date

5-1-2012

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Kinesiology and Nutrition Sciences

First Committee Member

Antonio Santo

Second Committee Member

John Young

Third Committee Member

Richard Tandy

Fourth Committee Member

Patricia Alpert

Number of Pages

74

Abstract

Background: Cardiovascular disease (CVD) represents a major cause of death in the United States, with abnormal levels of blood lipids and physical inactivity considered as major modifiable risk factors. The conventional lipid profile has been used to assess for CVD risk by directly measuring the concentrations of blood lipids. However, lipoprotein particle size and number obtained from a novel method, Nuclear Magnetic Resonance (NMR) may also asses for CVD risk with greater sensitivity. Exercise and increased physical activity has been shown to produce favorable effects on blood lipids and consequently reduce CVD risk. To understand this effect, it is important to understand the acute effects of an exercise session on these parameters. Purpose: The purpose of this study was to examine the effect of a 60-minute bout of dynamic exercise on lipoprotein particle number and size as measured by NMR, and compare it to the conventional lipid profile analysis. Method: Eight active healthy men between the ages of 19-34 years (age= 26 ± 5.17) participated in the study. After assessment of body composition and aerobic fitness, participants ran for 60 minutes at 70% of their aerobic fitness on a motor driven treadmill. Fasting blood samples were drawn immediately before, 5 minutes and 24 hours after exercise. Samples were sent to LipoScience, Inc. for the analysis of the lipoproteins by the NMR method and to Quest Diagnostics for the conventional lipid profile analysis. Results: The conventional profile showed a significant change in triglycerides (TG), (p=.019) immediately after exercise with a significant increase of 22%, then a non significant decrease of 13% from baseline after 24 hours. The NMR profile showed a significant change in the large HDL particle concentration (p=.046) with an increase of 5.8% observed immediately after exercise, and a decrease of 6.7% observed 24 hours after exercise. However, none of these changes were significantly different from the baseline value. Both profiles did not show any significant changes in any of the other parameters. Conclusion: Changes were observed in blood lipids that might be attributed to the session of exercise. The conventional profile has detected a significant change in HDL-C and TG. However, these changes were not significant from baseline. Also, the NMR profile detected changes in the HDL particles through large HDL particle concentration and this change in large HDL particle concentration was not significant from baseline either. So, the NMR profile was not more sensitive in detecting acute exercise-induced changes on blood lipids, in terms of CVD risk.

Keywords

Acute exercise; Blood lipids; Cardiovascular system—Diseases – Prevention; Exercise; Lipoproteins; Nuclear magnetic resonance (NMR)

Disciplines

Cardiovascular Diseases | Exercise Science | Kinesiology

Language

English


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