The Role of Temperature and Swab Materials in the Recovery of Herpes Simplex Virus from Lesions

Document Type



Swab specimens of lesions from patients attending a sexually transmitted disease clinic that were thought to be caused by HSV were cultured to determine whether recovery of HSV was influenced by the use of cotton swabs vs. calcium alginate swabs and by storage of the specimens at refrigerator temperature (4 C) vs. freezer temperature ( - 20 C). Adverse effects by both the use of calcium alginate swabs and the storage of specimens at freezing temperatures have been reported for laboratory-prepared stock cultures of HSV [2, 3] but not for clinical specimens.


Female Urogenital Diseases and Pregnancy Complications | Investigative Techniques | Male Urogenital Diseases | Public Health


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