Evaluation of Epifluorescence Methods for Quantifying Bioaerosols in Fine and Coarse Particulate Air Pollution
First page number:
Last page number:
Despite being recognized as an important part of particulate matter (PM) air pollution and health risk, bioaerosols have not been quantified as extensively as other PM components for establishing PM standards and management strategies. The challenge lies partly in the lack of practical measurement methods. This study evaluated a filter-based, direct-staining fluorescence microscopy (DS-FM) method that may be adapted to routine air quality monitoring for bioaerosol concentration and size distribution. Through testing with bioaerosol standards made of bacterial cells and fungal spores, the method is shown to have precision, accuracy, detection limit, and dynamic range suitable for most ambient environments. DS-FM was demonstrated with PM samples from an arid urban location in Las Vegas, Nevada during the spring allergy season... See full text for complete abstract.
Primary bbiological aerosol particles; Automated particle counting; Aerosol size distribution; DNA stain; PM10; PMcoarse
Environmental Health | Environmental Microbiology and Microbial Ecology
Chen, L. A.,
Chow, J. C.,
Patel, T. Y.,
Buttner, M. P.,
Watson, J. G.
Evaluation of Epifluorescence Methods for Quantifying Bioaerosols in Fine and Coarse Particulate Air Pollution.
Atmospheric Environment, 213