Location
University of Nevada, Las Vegas
Start Date
3-8-2010 9:00 AM
End Date
3-8-2010 12:00 PM
Description
Shigella flexneri is a gram negative non-motile, non-spore forming, rod-shaped bacterium responsible for bacillary dysentery in humans. The master regulator, VirF, initiates a cascade of virulence gene activation by acting as a transcription factor for the gene encoding the global regulator, VirB (1). Production of VirB is also negatively regulated by the regulatory small RNA (sRNA), RyhB 2). Regulatory sRNAs are untranslated RNA molecules involved in the regulation of both transcription and translation. RyhB, a 90 nt sRNA, was first identified in E. coli and subsequently found in all Shigella species. In Shigella this sRNA is maximally expressed in response to iron depletion and is responsible for the reduced expression of many virulence genes in Shigella flexneri by downregulating virB (2).
Keywords
Genetic regulation; Non-coding RNA; Shigella flexneri; Virulence (Microbiology)—Genetic aspects
Disciplines
Bacteriology | Genetics and Genomics | Life Sciences | Microbiology
Language
English
The Small regulatory RNA RyhB regulates icsA expression in Shigella flexneri
University of Nevada, Las Vegas
Shigella flexneri is a gram negative non-motile, non-spore forming, rod-shaped bacterium responsible for bacillary dysentery in humans. The master regulator, VirF, initiates a cascade of virulence gene activation by acting as a transcription factor for the gene encoding the global regulator, VirB (1). Production of VirB is also negatively regulated by the regulatory small RNA (sRNA), RyhB 2). Regulatory sRNAs are untranslated RNA molecules involved in the regulation of both transcription and translation. RyhB, a 90 nt sRNA, was first identified in E. coli and subsequently found in all Shigella species. In Shigella this sRNA is maximally expressed in response to iron depletion and is responsible for the reduced expression of many virulence genes in Shigella flexneri by downregulating virB (2).
Comments
Poster research sponsored by Department and Grant Supported Students