Comparative Analysis of Gene Expression Patterns for Oral Epithelium-Related Functions with Aging
Advances in Experimental Medicine and Biology
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Epithelial cells and functions of the epithelium are critical to the health of the oral cavity. We used a nonhuman primate model to profile the transcriptome of gingival tissues in health across the lifespan and hypothesized that in older animals, epithelial-related transcriptome patterns would reflect epithelial cells that are aggressively responsive to the surrounding environment and less able to modulate and resolve the noxious challenge from the bacteria. Rhesus monkeys (n = 34) with a healthy periodontium were distributed into four groups: ≤3 years (young), 3–7 years (adolescent), 12–16 years (adult), and 18–23 years (aged), and a buccal gingival sample from the premolar/molar region of each animal was obtained. RNA was subjected to a microarray analysis (GeneChip® Rhesus Macaque Genome Array, Affymetrix), and 336 genes examined that are linked to epithelium and epithelial cell functions categorized into 9 broad functional groups: extracellular matrix and cell structure; extracellular matrix remodeling enzymes; cell adhesion molecules, cytoskeleton regulation; inflammatory response; growth factors; kinases/cell signaling; cell surface receptors; junction associated molecules; autophagy/apoptosis; antimicrobial peptides; and transcription factors. (See full text for complete abstract)
Oral Biology and Oral Pathology
Ebersole, J. L.,
Novak, M. J.,
Gonzalez, O. A.
Comparative Analysis of Gene Expression Patterns for Oral Epithelium-Related Functions with Aging.
Advances in Experimental Medicine and Biology, 1197
Cham, Switzerland: Springer Nature.