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Wastewater surveillance for poliovirus is a useful tool for spatial surveillance and evaluation of immunization efficacy. Polio testing in wastewater has been conducted since the 1980s in Finland and Israel, and the virus was recently discovered in New York. With increasing concern regarding the vaccination of children against the virus, there has been worry that polio cases could once again present themselves in the Las Vegas Valley. By testing for the virus in wastewater, we can monitor any new cases or outbreaks that occur and inform authorities as to the location and viral count. The wastewater samples were collected at various locations throughout the Las Vegas Valley and brought to the lab for analysis. Nucleic acids from the samples were isolated and purified, and complementary DNA was for further testing. The DNA was tested using quantitative polymerase chain reaction to determine the concentration of poliovirus in the starting sample. After several months of testing, no polio was identified in the wastewater. The qPCR data indicated that no poliovirus was replicated. This data is useful moving forward because it reveals that there were no major polio outbreaks in the areas that were tested. Further research would need to be conducted in order to ensure continuing safety from poliovirus in Las Vegas.
Las Vegas (Nev.)
University of Nevada, Las Vegas
Viral pollution of water; Water--Testing
Viruses | Water Resource Management
Boss, Sage; Baker, Hayley; and Chang, Ching-Lan, "Poliovirus Detection in Las Vegas Wastewater" (2022). Undergraduate Research Symposium Posters. 139.
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Faculty Mentors: Edwin C. Oh, Van Vo