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Description
Milk was historically thought to be generally sterile. However, use of culture-dependent and culture-independent methods has provided strong evidence that milk has a microbiome (Figure 1). Culture-independent methods (e.g., high-throughput sequencing) have demonstrated the diversity of microbes in milk but provide relative abundance data. Culture-dependent methods have indicated microbial communities in milk are of low abundance, but it is challenging to measure absolute quantities accurately. Real-time quantitative polymerase chain reaction (RT-qPCR) has been used to quantify bacterial taxa in milk, However, concentrations often fall below the detection limit (Figure 2 & 3). Digital droplet PCR (ddPCR) allows detection of low-abundant copies of DNA due to its high sensitivity (Figure 4).
Publisher Location
Las Vegas (Nev.)
Publication Date
Fall 12-8-2023
Publisher
University of Nevada, Las Vegas
Language
English
Controlled Subject
Milk--Microbiology; Bacteria; Polymerase chain reaction
Disciplines
Bacteria | Microbiology
File Format
File Size
1470 KB
Recommended Citation
Chavez, Carmina; Williams, Janet E.; McGuire, Michelle K.; and McGuire, Mark A., "Comparison of Molecular Methods for Absolute Quantification of Low-Abundant Bacterial Communities in Milk" (2023). Undergraduate Research Symposium Posters. 198.
https://digitalscholarship.unlv.edu/durep_posters/198
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IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/
Comments
Faculty Mentor: Mark McGuire