The Classical Pathway as a Function of Temperature in Hibernation Stages, in Tenrec ecaudatus
For this study, a competitive hemolysis assay was used to visualize how the complement proteins found in tenrec serum competed against the anti-sheep rabbit hemolysin in which the target cells were 100% sheep erythrocytes. The assay was ran at an absorbance of 540nm in order to detect lysis. The HEPES buffer and total lysis buffer were used as controls in order to take 100% lysis and background lysis into consideration of the system. This study was used to gather preliminary data, and will be used for further research in understanding the ambiguity of innate protein immunity in tenrecs.