Myocardial infarction; LV remodeling; Intramyocardial hemorrhage; Cardiomyocyte; mTOR; Insulin
Medicine and Health Sciences
When an acute myocardial infarction (MI) occurs, the heart becomes ischemic. Medical treatments such as stents have improved the recovery process after a MI, but there is still a high risk for heart failure. Due to the resulting intramyocardial hemorrhage, residual hemoglobin with excess iron compromises cardiomyocyte (CM) survival. Previous studies suggest that the magnitude of CM cell death is directly proportional to the level of adverse left ventricular (LV) remodeling. Mechanistic target of rapamycin (mTOR) is a key downstream signaling pathway that is sufficient for CM cell survival against iron and responds to insulin, a cardioprotective growth factor. However, the effect of insulin in excess iron-induced cell death in CMs is not well characterized. Using H9c2 cardiomyoblasts, originally derived from embryonic rat ventricle cells, the effects of insulin in CM cell survival against excess iron were examined. The cells were pre-treated with varying dosages of insulin before applying iron (III) citrate. Cell viability was assessed by Live/Dead Assay, in which live cells stain with calcein AM (green) and nuclei of dead cells stain with ethidium homodimer-1 (red). In comparison to the amount of cell death caused by iron alone, insulin decreased dead cell count substantially. The greatest concentration of 1µM of insulin with iron resulted in a statistical significance of p<0.02 (n=3-4). The results indicate that insulin has the potential to mediate iron-induced CM death. Understanding the effect of insulin as a combatant of iron-induced cell death with an intramyocardial approach would lead to better therapeutic preventions of heart failure.
Tanaka, Carina and Matsui, M.D., Ph.D., FAHA, Takashi
"Protective Effects of Insulin in Cardiomyocytes Against Iron-mediated Cell Death,"
Journal of Health Disparities Research and Practice: Vol. 12
, Article 27.
Available at: https://digitalscholarship.unlv.edu/jhdrp/vol12/iss4/27