Protein Expression of Steroid Receptors in Macaca mulatta endometriotic Lesions

Authors

Héctor Torres
Lynette Ruiz, PhD, Ponce Health Sciences University
Olga Gonzalez, DVM, Caribbean Primate Research Center-UPR
Luisa Morales, DrPH, Ponce Health Science University

Keywords

Endometriosis; rhesus macaques; steroid receptors; non-human primates; immunohistochemistry

Disciplines

Bilingual, Multilingual, and Multicultural Education | Community College Leadership | Higher Education | Immune System Diseases | Medicine and Health Sciences | Public Health | Translational Medical Research | Virus Diseases

Abstract

Endometriosis is pathologically defined as the presence of endometrial tissue in ectopic locations. Currently, there are no specific, non-invasive biomarkers and no cure for endometriosis. Non-human primates (NHP), including Macaca mulatta (rhesus macaques), have been highlighted as appropriate models to study endometriosis due to genetic, physiological, and anatomical similarities to humans. Research shows endometriosis might be associated with molecular deviations in human peritoneal endometriosis, such as increased levels of estrogen receptors and decreased levels of progesterone receptors.

The aim of this study is to determine protein expression of steroid receptors in five endometriotic lesions of rhesus macaques. We propose that lesions of rhesus macaques will express elevated estrogen receptors and diminished progesterone receptors, similar to human endometriotic lesions. Endometriotic lesions of rhesus macaques were obtained at time of surgery from different locations, and steroid receptor expression was determined by immunohistochemistry. This study was able to determine the expression of steroid receptors in endometriotic lesions of rhesus macaques; however the results were not able to support our hypothesis. Results did not observe significant differences between estrogen and progesterone receptor protein expressions in glandular and stromal compartments of the endometriotic lesions. Still, this animal model represents a valuable tool to study endometriosis since they do develop spontaneous endometriosis. Future studies should match the location of lesions and classify the severity of endometriosis in the rhesus macaques.

Recommended Citation

Torres, Héctor; Ruiz, PhD, Lynette; Gonzalez, DVM, Olga; and Morales, DrPH, Luisa (2016) "Protein Expression of Steroid Receptors in Macaca mulatta endometriotic Lesions," Journal of Health Disparities Research and Practice: Vol. 9: Iss. 5, Article 98.
Available at: https://digitalscholarship.unlv.edu/jhdrp/vol9/iss5/98