Multiplexed Colorimetric Detection of SARS-CoV-2 and Other Pathogens in Wastewater on a 3D Printed Integrated Microfluidic Chip

Authors

Kun Yin, UConn Health
Xiong Ding, UConn Health
Zhiheng Xu, UConn Health
Ziyue Li, UConn Health
Xingyu Wang, University of Connecticut
Hui Zhao, University of Nevada, Las VegasFollow
Clifford Otis, University of Connecticut
Baikun Li, University of Connecticut
Changchun Liu, UConn Health

Document Type

Article

Publication Date

6-7-2021

Publication Title

Sensors and Actuators B: Chemical

Volume

344

First page number:

1

Last page number:

10

Abstract

Severe acute respiratory coronavirus 2 (SARS-CoV-2) pandemic has become a global public health emergency. The detection of SARS-CoV-2 and human enteric pathogens in wastewater can provide an early warning of disease outbreak. Herein, a sensitive, multiplexed, colorimetric detection (termed “SMCD”) method was established for pathogen detection in wastewater samples. The SMCD method integrated on-chip nucleic acid extraction, two-stage isothermal amplification, and colorimetric detection on a 3D printed microfluidic chip. The colorimetric signal during nucleic acid amplification was recorded in real-time and analyzed by a programmed smartphone without the need for complicated equipment. By combining two-stage isothermal amplification assay into the integrated microfluidic platform, we detected SARS-CoV-2 and human enteric pathogens with sensitivities of 100 genome equivalent (GE)/mL and 500 colony-forming units (CFU)/mL, respectively, in wastewater within one hour. Additionally, we realized smart, connected, on-site detection with a reporting framework embedded in a portable detection platform, which exhibited potential for rapid spatiotemporal epidemiologic data collection regarding the environmental dynamics, transmission, and persistence of infectious diseases.

Keywords

3D printed integrated microfluidic chip; Colorimetric detection; Human enteric pathogens; SARS-CoV-2; Smartphone-based detection platform

Disciplines

Biomedical Devices and Instrumentation | Biomedical Engineering and Bioengineering

Language

English

Repository Citation

Yin, K., Ding, X., Xu, Z., Li, Z., Wang, X., Zhao, H., Otis, C., Li, B., Liu, C. (2021). Multiplexed Colorimetric Detection of SARS-CoV-2 and Other Pathogens in Wastewater on a 3D Printed Integrated Microfluidic Chip. Sensors and Actuators B: Chemical, 344 1-10.
http://dx.doi.org/10.1016/j.snb.2021.130242