Multiplexed Colorimetric Detection of SARS-CoV-2 and Other Pathogens in Wastewater on a 3D Printed Integrated Microfluidic Chip
Sensors and Actuators B: Chemical
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Severe acute respiratory coronavirus 2 (SARS-CoV-2) pandemic has become a global public health emergency. The detection of SARS-CoV-2 and human enteric pathogens in wastewater can provide an early warning of disease outbreak. Herein, a sensitive, multiplexed, colorimetric detection (termed “SMCD”) method was established for pathogen detection in wastewater samples. The SMCD method integrated on-chip nucleic acid extraction, two-stage isothermal amplification, and colorimetric detection on a 3D printed microfluidic chip. The colorimetric signal during nucleic acid amplification was recorded in real-time and analyzed by a programmed smartphone without the need for complicated equipment. By combining two-stage isothermal amplification assay into the integrated microfluidic platform, we detected SARS-CoV-2 and human enteric pathogens with sensitivities of 100 genome equivalent (GE)/mL and 500 colony-forming units (CFU)/mL, respectively, in wastewater within one hour. Additionally, we realized smart, connected, on-site detection with a reporting framework embedded in a portable detection platform, which exhibited potential for rapid spatiotemporal epidemiologic data collection regarding the environmental dynamics, transmission, and persistence of infectious diseases.
3D printed integrated microfluidic chip; Colorimetric detection; Human enteric pathogens; SARS-CoV-2; Smartphone-based detection platform
Biomedical Devices and Instrumentation | Biomedical Engineering and Bioengineering
Multiplexed Colorimetric Detection of SARS-CoV-2 and Other Pathogens in Wastewater on a 3D Printed Integrated Microfluidic Chip.
Sensors and Actuators B: Chemical, 344