Title

Structural Mechanisms of Acute VEGF Effect on Microvessel Permeability

Document Type

Article

Publication Date

6-2003

Publication Title

American Journal of Physiology-Heart and Circulatory Physiology

Volume

284

Issue

6

First page number:

H2124

Last page number:

H2135

Abstract

To investigate the ultrastructural mechanisms of acute microvessel hyperpermeability by vascular endothelial growth factor (VEGF), we combined a mathematical model (J Biomech Eng 116: 502–513, 1994) with experimental data of the effect of VEGF on microvessel hydraulic conductivity (L p) and permeability of various-sized solutes. We examined the effect of VEGF on microvessel permeability to a small solute (sodium fluorescein, Stokes radius 0.45 nm), an intermediate solute (α-lactalbumin, Stokes radius 2.01 nm), and a large solute [albumin (BSA), Stokes radius 3.5 nm]. Exposure to 1 nM VEGF transiently increased apparent permeability to 2.3, 3.3, and 6.2 times their baseline values for sodium fluorescein, α-lactalbumin, and BSA, respectively, within 30 s, and all returned to control within 2 min. On the basis ofL p (DO Bates and FE Curry. Am J Physiol Heart Circ Physiol 271: H2520–H2528, 1996) and permeability data, the prediction from the model suggested that the most likely structural changes in the interendothelial cleft induced by VEGF would be a ∼2.5-fold increase in its opening width and partial degradation of the surface glycocalyx.

Keywords

Mathematical models; Tissues – Permeability; Vascular endothelial growth factors

Disciplines

Biomechanics and Biotransport | Biomedical Engineering and Bioengineering | Molecular, Cellular, and Tissue Engineering

Language

English

Permissions

Use Find in Your Library, contact the author, or interlibrary loan to garner a copy of the item. Publisher policy does not allow archiving the final published version. If a post-print (author's peer-reviewed manuscript) is allowed and available, or publisher policy changes, the item will be deposited.

Identifier

DOI: 10.1152/ajpheart.00894.2002

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