M1/70 attenuates blood-borne neutrophil oxidants, activation and myofiber damage following stretch injury
The purpose of this study was to determine the role of the CD11b-dependent respiratory burst in neutrophil oxidant generation and activation, interleukin-8 (IL-8) production, and myofiber damage after muscle stretch injury by using the monoclonal antibody M1/70 to block this pathway. Twelve male New Zealand White rabbits were randomly assigned to a treatment group: M1/70 (n = 6), IgG isotype control (n = 3), or saline control (n = 3). After intravenous injection of the assigned agent under gas anesthesia, a standardized single-stretch injury was created in the right tibialis anterior, whereas the left tibialis anterior underwent a sham surgery. Blood-borne neutrophil oxidant generation and CD11b receptor density and plasma IL-8 levels were measured pre- and 24 h postinjury. Damage was assessed histologically at the hematoma site by counting torn myofibers. M1/70 group demonstrated decreased blood-borne neutrophil oxidant generation (P < 0.05) and CD11b receptor density (P < 0.05), an increase in plasma IL-8 concentration (P < 0.01), and less torn myofibers (P < 0.01) compared with IgG isotype or saline control groups. These data indicate that 1). CD11b-dependent respiratory burst is a major source of oxidants produced by the neutrophil, and that treatment with M1/70 2). attenuates neutrophil activation status, 3). increases plasma IL-8 concentration, and 4). minimizes myofiber damage 24 h postmuscle stretch injury.
Animals; Antibodies; Monoclonal/pharmacology; Antigens; CD11b/metabolism; Antigens; CD11b/physiology; Cell Count; Cell Survival; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Immunohistochemistry; Interleukin-8/blood; Leukocyte Count; Male; Muscle Fibers; Skeletal/pathology; Muscle; Skeletal/injuries; Muscle; Skeletal/pathology; Neutrophil Activation/drug effects; Neutrophils/drug effects; Neutrophils/metabolism; Oxidants/metabolism; Rabbits; Respiratory Burst/drug effects; Tendon Injuries/pathology
Medicine and Health Sciences | Nursing
Ji, L. L.,
Schneider, B. S.,
Best, T. M.
M1/70 attenuates blood-borne neutrophil oxidants, activation and myofiber damage following stretch injury.
Journal of Applied Physiology, 95(3),