Title

CD11b+ neutrophils predominate over RAM11+ macrophages in stretch-injured muscle

Document Type

Article

Abstract

The purpose of this study was to determine whether both neutrophils and macrophages infiltrate the hematoma site of stretch-injured rabbit tibialis anterior muscle. The Mab.198 antibody was used to detect CD11b(+) neutrophils or macrophages. Neutrophils were identified specifically by using the RPN3/57 antibody. The RAM11 antibody was used to detect macrophages. The histological characteristics of the hematoma site, torn fibers or inflammatory cells, were present primarily at 4 and 24 h, but not at 48 and 72 h after injury. A difference in the Mab.198(+) cellular concentration was detected over time between uninjured and injured muscles (P = 0.03). The injured-uninjured difference in the RPN3/57(+) or RAM11(+) cellular concentrations approached significance (P = 0.07) or else was deemed insignificant (P = 0.13), respectively. Therefore, neutrophils may predominate over RAM11(+) macrophages in stretch-injured muscle. These findings may influence the antiinflammatory strategies used to treat stretch injuries.

Keywords

Animals; Antigens; Anti-inflammatory agents; Differentiation/biosynthesis; Biological Markers/analysis; Cell Count; Disease Models; Animal; Disease Progression; Hematoma; Hematoma/pathology; Immunohistochemistry; Macrophage-1 Antigen/biosynthesis; Macrophages; Macrophages/metabolism; Macrophages/pathology; Muscle; Skeletal/pathology; Muscle; Skeletal/physiopathology; Muscles – Wounds and injuries; Neutrophil Infiltration; Neutrophils; Neutrophils/metabolism; Neutrophils/pathology; Rabbits – Wounds and injuries; Sprains and Strains/pathology; Sprains and Strains/physiopathology; Stress; Mechanical; Torque

Disciplines

Kinesiology | Medicine and Health Sciences | Nursing

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