Title

Isolation and characterization of microsatellite markers in the lowland leopard frog (Rana yavapaiensis) and the relict leopard frog (R. onca), two declining frogs of the North American desert southwest

Document Type

Article

Publication Date

1-2009

Publication Title

Molecular Ecology Resources

Volume

9

Issue

1

First page number:

199

Last page number:

202

Abstract

We characterized 15 microsatellite loci for the lowland leopard frog (Rana yavapaiensis) and the relict leopard frog (R. onca) for future studies of population genetic structure and relatedness. Analysis of 20 individuals from single populations of each species showed that all markers were polymorphic in at least one species. Observed and expected heterozygosities ranged from 0 to 0.94 and from 0.11 to 0.85, respectively, and there were three to 11 alleles per locus. No loci were in linkage disequilibrium, but six loci deviated significantly from Hardy–Weinberg equilibrium, and the presence of a null allele was detected in two of these loci.

Keywords

Anuran; Microsatellite; Polymorphism; Rana onca; Rana yavapaiensis

Disciplines

Animal Sciences | Biology | Environmental Sciences | Life Sciences | Molecular Biology | Zoology

Language

English

Permissions

Use Find in Your Library, contact the author, or interlibrary loan to garner a copy of the item. Publisher policy does not allow archiving the final published version. If a post-print (author's peer-reviewed manuscript) is allowed and available, or publisher policy changes, the item will be deposited.

Identifier

DOI: http://dx.doi.org/10.1111/j.1755-0998.2008.02343.x

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