Master of Science (MS)
First Committee Member
Number of Pages
We attempted to clone closely-related members of the human heat shock protein 27 (hsp 27) multigene family. Genomic DNA was obtained from human A549 lung cancer cells. Colony hybridization and the Polymerase chain reaction identified the hsp 27 genes. Positive clones were subcloned into pGEM-3Z. The DNA sequence information for three of the clones was determined, and analyzed using DNAsis and Blast Algorithms. DNA Alignments of the inserts contained in three positive clones showed a homology of 29-51% to the human hsp 27 gene. Our data suggests that these inserts are distant members of the human hsp 27 multigene family. Blast searches did not determine the identity of these inserts in GenBank and SwissProt databases (11/1/98). This result indicated that the inserts are novel sequences. Hence, the nucleotide information for the inserts was submitted to GenBank (Accession Numbers: AF120329, AF120330, AF120331, AF120332, and AF120333).
Cloning; Family; Heat; Human; Multigene; Protein; Seven; Shock; Towards; Twenty
Biochemistry; Cellular biology; Molecular biology
University of Nevada, Las Vegas
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Ohiaeri, Ann A, "Towards the cloning of the human heat shock protein twenty-seven multigene family" (1999). UNLV Retrospective Theses & Dissertations. 1007.