Award Date

1-1-2003

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry

First Committee Member

Stephen W. Carper

Number of Pages

86

Abstract

Human breast cancer cell lines DC4, DB46, MCF-7, MDA-MD-435 were incubated with cytochrome c at 5 x 10-7M, 5 x 10 -6M and 5 x 10-5M. Human colon cancer cell line, RKO, and normal human lung fibroblast cell line, HFL1, were incubated with cytochrome c at 5.6 x 10-8M, 5.6 x 10-7M and 5.6 x 10-6M. Cytochrome c exposure showed a dose response including cell death in breast cancer cell lines. RKO showed no sensitivity to cytochrome c exposure at any of the concentrations and HFL1 showed sensitivity only at the highest concentration. FAD, NADH, Q10 and ATP were incubated with cells at 1 x 10 -9M, 1 x 10-8M and 1 x 10 -7M. Incubation of FAD with DC4 and DB46 was also done at 5 x 10-7M, 5 x 10-6M and 5 x 10-5M. FAD, ATP and NADH were more effective in cell death induction at much lower concentrations than cytochrome c. Q 10 was least effective in cell death induction.

Keywords

Breast; Cancer; Carriers; Cell; Cytotoxic; Effects; Electron Inhibited; Lines

Controlled Subject

Biochemistry; Oncology

File Format

pdf

File Size

2334.72 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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