Award Date

1-1-2005

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Chemistry

First Committee Member

Stephen W. Carper

Number of Pages

63

Abstract

Methyl jasmonate (MJ) and cis jasmone (CJ), lipid-derived plant stress hormones, were studied for their effects against both hormone dependent (LNCaP) and hormone independent (PC3) human prostate cancer cell lines. Both 2 mM MJ or CJ inhibited the growth of the cell lines. An alamar blue assay was used to determine IC50 values for MJ and CJ in both cell lines at 24 hrs, 48 hrs and 72 hrs. At 24 hrs the MJ IC50 values were 2.25 and 2.05 while the CJ values were 3.00 and 1.25 in the PC3 and LNCaP lines respectively. Cell cycle analysis revealed that MJ and CJ induced apoptosis in both cell lines as well as activated caspase-3. An in vitro assay showed that MJ and CJ did not significantly inhibit either 5-lipoxygenase or 15-lipoxygenase. 5-HETE was able to stimulate cell growth in the presence or absence of MJ in both the cell lines.

Keywords

Apoptosis; Cancer; Jasmonate; Jasmone; Lines; Methyl Prostate

Controlled Subject

Biochemistry

File Format

pdf

File Size

1310.72 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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