Award Date

1-1-2008

Degree Type

Thesis

Degree Name

Master of Science (MS)

First Committee Member

Steen J. Madsen

Number of Pages

40

Abstract

The F98 rat glioma cell line, a model for human malignant glioma, was used to investigate the efficacy of 5-aminolevulinic acid (ALA)-mediated photodynamic therapy (PDT) under varying light irradiation conditions. Cells in monolayer were pre-incubated in ALA for 4 hours prior to 635 nm light exposure. Using clonogenic survival assays, it was found that F98 glioma cells were sensitive to ALA-PDT: survival was inversely proportional to light fluence. As evidenced from the LD50 (4 J cm-2 at a fluence rate of 35 mW cm-2), ALA-PDT was found to be more effective in F98 cells compared to that found in another commonly used rat glioma cell line (BT4C). In contrast to findings in BT4C cells, F98 survival was independent of fluence rate; ALA-PDT was tested for its ability to enhance the cytotoxicity of Bleomycin (BLM), a procedure termed photochemical internalization (PCI). F98 cells were incubated in ALA and BLM prior to illumination. In all cases, no difference in survival was observed between F98 cells subjected to ALA-PCI delivery of BLM and cells treated with ALA-PDT. These findings suggest that ALA is not an ideal compound for PCI applications.

Keywords

Acid; Aminolevulinic; Based; Bleomycin; Cell; Effects; Glioma; Internalization; Line; Photochemical; Photodynamic; Rat; Therapy

Controlled Subject

Biophysics; Oncology

File Format

pdf

File Size

0.77 MB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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