Award Date


Degree Type


Degree Name

Doctor of Philosophy (PhD)


Civil and Environmental Engineering

First Committee Member

Jacimaria Ramos Batista

Number of Pages



The impacts of different carbon sources, particularly VFAs, on EBPR's microbial population were investigated using T-RFLP and FISH. System performance and both general and specific microbial changes were investigated in SBR systems fed with acetate, propionate, butyrate, valerate, and glucose. Acetate and a mixture of acetate and propionate promoted the best P-removal. Butyrate and valerate lead the systems to fail in the long run. Glucose and propionate promoted poor EBPR, but the performance of the system fed with propionate improved with time. T-RFLP results indicated that short carbon chain VFAs, long carbon chain VFAs, and non-VFA favored different microbial populations in the systems. FISH results revealed that acetate and propionate successfully selected for the PAO, Candidatus accumulibacter phosphatis, while the other substrates tested did not. FISH analysis also showed that the GAO, Candidatus competibacter phosphatis, could not survive under any of the carbon feedings investigated, except under a feed containing a mixture of acetate and propionate.


Acids; Bacterial; Biological; Different; Enhanced; Fatty; Impact; Phosphorus; Phosphorus Removal; Selection; Systems; Types; Volatile; Volatile Fatty Acids

Controlled Subject

Environmental engineering

File Format


File Size

8448 KB

Degree Grantor

University of Nevada, Las Vegas




If you are the rightful copyright holder of this dissertation or thesis and wish to have the full text removed from Digital Scholarship@UNLV, please submit a request to and include clear identification of the work, preferably with URL.


IN COPYRIGHT. For more information about this rights statement, please visit