Award Date

1-1-1989

Degree Type

Thesis

Degree Name

Master of Science (MS)

First Committee Member

Stanley D. Hillyard

Number of Pages

90

Abstract

The amphibian antidiuretic hormone arginine vasotocin (AVT) stimulated osmotic water flow (JH{dollar}\sb2{dollar}O) across isolated skin from the pelvic but not the pectoral region of the toad, Bufo woodhousei. The apical membrane capacitance (C{dollar}\sb{\rm a}{dollar}) of the cells in the stratum granulosum of neither skin region was changed by AVT treatment when an osmotic gradient was present across the tissue; Short circuit current (I{dollar}\sb{\rm sc}{dollar}) and the amiloride sensitive current (I{dollar}\sb{\rm Na\sp+}{dollar}) both increased significantly in the pelvic and in the pectoral skin after AVT treatment, but to a larger degree in the pelvic skin. If M is expressed as a function of C{dollar}\sb{\rm a}{dollar}, the density of Na{dollar}\sp+{dollar} channels (assuming 1{dollar}\mu{dollar}F/cm{dollar}\sp2{dollar}) was 90 channels/{dollar}\mu{dollar}m{dollar}\sp2{dollar} of actual cell membrane area before AVT treatment and 273 channels/{dollar}\mu{dollar}m{dollar}\sp2{dollar} after AVT treatment. In the pectoral skin M did not increase significantly, however, I{dollar}\sb{\rm Na\sp+}{dollar} was linearly related to M. The current through single Na{dollar}\sp+{dollar} channels (i) did not change in either skin region after AVT treatment. (Abstract shortened with permission of author.).

Keywords

Bufo woodhousei; Capacitance; Channel; Comparison; Densities; Flux; Ion; Membrane Pectoral; Pelvic Skin; Sodium; Toad; Water; Woodhouse; AVT

Controlled Subject

Zoology; Biochemistry

File Format

pdf

File Size

2.17 MB

Degree Grantor

University of Nevada, Las Vegas

Language

English

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