Master of Science (MS)
First Committee Member
Carl L. Reiber
Number of Pages
I hypothesized that experimental elimination of blood flow via ligation of the conotruncus arteriosus would cause a cessation of blood vessel development and even vessel regression. India ink, injected to highlight the vasculature, had no effect on blood flow. Embryos were divided into ligated, sham, and control groups. After ligature placement and 6 hr. reincubation, KCl, added topically induced cardiac arrest. The diameters of the anterior cardinal vein, anterior vitelline vein, vitelline artery, and vitelline vein were not statistically different from sham and control values. Eye diameter increased equally in the presence and absence of blood flow. These results demonstrate that blood flow had no influence on vessel growth in diameter during a 6 hr. period. Blood flow did appear to offer a stabilizing influence against vessel degradation. A critical window of 6 hr. may exist during which the absence of blood flow has no detrimental effects on embryonic vasculature.
Angiogenesis; Blood; Chick; Day; Embryo; Flow; Influence
University of Nevada, Las Vegas
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Clarke, Brett Mark, "The influence of blood flow on angiogenesis in the 3-day chick embryo" (1998). UNLV Retrospective Theses & Dissertations. 955.