Document Type
Article
Publication Date
10-7-2021
Publication Title
Wound Repair and Regeneration
First page number:
1
Last page number:
18
Abstract
Negative pressure wound therapy (NPWT) is used clinically to promote tissue formation and wound closure. In this study, a porcine wound model was used to further investigate the mechanisms as to how NPWT modulates wound healing via utilization of a form of NPWT called the vacuum-assisted closure. To observe the effect of NPWT more accurately, non-NPWT control wounds containing GranuFoam™ dressings, without vacuum exposure, were utilized. In situ histological analysis revealed that NPWT enhanced plasma protein adsorption throughout the GranuFoam™, resulting in increased cellular colonization and tissue ingrowth. Gram staining revealed that NPWT decreased bacterial dissemination to adjacent tissue with greater bacterial localization within the GranuFoam™. Genomic analysis demonstrated the significant changes in gene expression across a number of genes between wounds treated with non-NPWT and NPWT when compared against baseline tissue. However, minimal differences were noted between non-NPWT and NPWT wounds, including no significant differences in expression of collagen, angiogenic, or key inflammatory genes. Similarly, significant increases in immune cell populations were observed from day 0 to day 9 for both non-NPWT and NPWT wounds, though no differences were noted between non-NPWT and NPWT wounds. Furthermore, histological analysis demonstrated the presence of a foreign body response (FBR), with giant cell formation and encapsulation of GranuFoam™ particles. The unique in situ histological evaluation and genomic comparison of non-NPWT and NPWT wounds in this pilot study provided a never-before-shown perspective, offering novel insights into the physiological processes of NPWT and the potential role of a FBR in NPWT clinical outcomes.
Keywords
Biomedical engineering; Foreign body response; Genomics; Negative pressure wound therapy; Porcine model; Wound healing
Disciplines
Biomedical Engineering and Bioengineering
File Format
File Size
26350 KB
Language
English
Rights
IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Repository Citation
Hodge, J. G.,
Pistorio, A. L.,
Neal, C. A.,
Dai, H.,
Nelson-Brantley, J. G.,
Steed, M. E.,
Korentager, R. A.,
Zamierowski, D. S.,
Mellott, A. J.
(2021).
Novel Insights Into Negative Pressure Wound Healing From an in Situ Porcine Perspective.
Wound Repair and Regeneration
1-18.
http://dx.doi.org/10.1111/wrr.12971