Doctor of Philosophy in Psychology
First Committee Member
Laurel M. Pritchard
Second Committee Member
Third Committee Member
Fourth Committee Member
Number of Pages
Methamphetamine (METH) abuse impacts the global economy through costs associated with drug enforcement, emergency room visits, and treatment. Hyperthermia is a leading cause of METH induced emergency room visits and may lead to neural damage. Previous research has demonstrated early life stress, such as childhood abuse, increases the likelihood of developing a substance abuse disorder. However, the effects of early life stress on neuronal damage induced by chronic METH administration are unknown. We aimed to elucidate the effects of early life stress on METH induced dopamine damage in the striatum. Animals were separated three hours per day during the first two weeks of development or 15 minutes for control. In adulthood, rats received either a subcutaneous 0.9% saline or 5.0 mg/kg METH injection every two hours for a total of four injections. Rectal temperatures were taken before the first injection and one hour after each subsequent injection. Seven days after testing, rats were euthanized and striatum was collected for quantification of tyrosine hydroxylase (TH) and dopamine transporter (DAT) content by Western blot. METH significantly elevated core body temperature in males and decreased striatal DAT and TH content and this effect was potentiated by early life stress. Females did not exhibit an effect of METH except in the elevated heat condition in the preliminary study, which significantly decreased DAT levels. We further ran a preliminary study looking at early life stress, METH dosing in adulthood during ambient (22-23°C) or elevated (27-30°C) temperature .Preliminary results indicated a replication of experiment one with no effect of elevated temperatures. These studies indicate maternal separation increases METH induced damage in males, and females are less susceptible to METH induced damage.
Brain damage; Corpus striatum; Methamphetamine; Methamphetamine abuse; Stress (Physiology)
Hensleigh, Emily, "The Effect of Early Life Stress on Methamphetamine Damage in the Striatum" (2014). UNLV Theses, Dissertations, Professional Papers, and Capstones. 2092.