Master of Science (MS)
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Drosophila melanogaster selected for resistance to desiccation (no food or water) display slower development and a higher body mass compared to fed controls due to an extended third larval instar. I hypothesize that desiccation selected D. melanogaster larvae will have a different gene expression profile compared to fed controls. Separate populations of D. melanogaster were subjected to desiccation (no food or water), starvation (no food) until 80-85% mortality, for 75 generations. mRNA from the larval fat body was collected at 88 hours, 96 hours, 112 hours and 120 hours post-hatching. Four replicate samples were used for each condition and time point. Gene expression was measured using two color cDNA microarrays. I analyzed the microarray data using a two-way factorial design ANOVA implemented in R using a significance level of 0.05 with FDR correction. The FDR adjusted results showed 43 genes differentially expressed for selection condition, 4590 genes for time, and 122 for selection by time (interaction). I then used DAVID 6.7 to explore which biological pathways are over-represented for these genes. One biological process was shown to be over-represented for the selection genes, 67 for time, and 10 for interaction. These over-represented processes suggest desiccation selected and control populations are indeed developing differently.
desiccation selection; differential gene expression; Drosophila melanogaster; larval fat body
Biology | Genetics
University of Nevada, Las Vegas
Charles, Adriana Michelle, "Gene Expression Profiling in the Larval Fat Body of Desiccation Selected Drosophila melanogaster" (2017). UNLV Theses, Dissertations, Professional Papers, and Capstones. 2956.
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