Location

University of Nevada Las Vegas, Student Union Ball Room

Start Date

6-8-2009 9:30 AM

End Date

6-8-2009 12:00 PM

Description

Our goal is to visualize IcsP on the surface of S. flexneri and determine how its localization changes during growth versus stationary phases. We hypothesize IcsP will be present on the surface of S. flexneri differentially during growth and stationary phases of cell cycle. We hypothesize greater levels of IcsP seen during stationary phase (simultaneous with lower, more unipolar IcsA, due to IcsP mediated cleavage). During growth phase, we expect less IcsP to be present on the surface, and IcsA to be more evenly distributed around the cell surface.

Keywords

Growth phases; IcsP; Proteases; Shigella flexneri; Stationary phases

Disciplines

Microbiology

Language

English

Comments

Abstract & poster


Included in

Microbiology Commons

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Aug 6th, 9:30 AM Aug 6th, 12:00 PM

Differential localization of IcsA and IcsP in the outer membrane of Shigella flexneri

University of Nevada Las Vegas, Student Union Ball Room

Our goal is to visualize IcsP on the surface of S. flexneri and determine how its localization changes during growth versus stationary phases. We hypothesize IcsP will be present on the surface of S. flexneri differentially during growth and stationary phases of cell cycle. We hypothesize greater levels of IcsP seen during stationary phase (simultaneous with lower, more unipolar IcsA, due to IcsP mediated cleavage). During growth phase, we expect less IcsP to be present on the surface, and IcsA to be more evenly distributed around the cell surface.