One-step Fabrication of Sub-10-nm Plasmonic nanogaps for Reliable SERS Sensing of Microorganisms
Document Type
Article
Publication Date
6-15-2013
Publication Title
Biosensors and Bioelectronics
Volume
44
First page number:
191
Last page number:
197
Abstract
Nanoscale gaps in noble metal films can produce intense electromagnetic enhancement. When Raman-active molecules are positioned in these regions, their surface-enhanced Raman scattering (SERS) signals can be dramatically enhanced. However, the lack of convenient and reliable fabrication methods with ultrasmall nanogaps (<10 nm) severely block the application of SERS. Here, we propose a cost-effective and reproducible technique to fabricate the large-area Ag SERS-active substrates which are full of the high-density, sub-10-nm nanogaps by high pressure sputtering, and the enhancement factor (EF) is testified to improve by 10(3) times compared to the continuous Ag film with a smooth surface (the roughness is 0.5 nm) and without nanogaps. Since there are no chemicals used during fabrication, this substrate has a clean surface, which is crucial for acquiring reliable SERS spectra. This SERS-active substrate has then been applied to identify a series of microorganisms, and excellent, reproducible SERS spectra were obtained. Finally, a set of piecewise-linear equations is provided according to the correlation between SERS intensity and rhodamine 6G (R6G) concentration, and the detection limit is calculated to be 0.2×10(-8)M. These results suggest that the high pressure sputtering is an excellent, reliable technique for fabricating sub-10-nm plasmonic nanogaps, and the SERS-based methodology is very promising for being used in biological sensing field.
Disciplines
Electrical and Computer Engineering | Electrical and Electronics | Electronic Devices and Semiconductor Manufacturing | Nanotechnology Fabrication | Power and Energy
Language
English
Permissions
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Repository Citation
Chen, J.,
Qin, G. W.,
Wang, J.,
Yu, J.,
Shen, B.,
Li, S.,
Ren, Y.,
Zuo, L.,
Shen, W.,
Das, B.
(2013).
One-step Fabrication of Sub-10-nm Plasmonic nanogaps for Reliable SERS Sensing of Microorganisms.
Biosensors and Bioelectronics, 44
191-197.