Development of a New Polymerase Chain Reaction Assay for the Rapid Detection of the Oral Pathogenic Bacterium, Selenomonas Noxia

Authors

Patrica Cruz-Perez, University of Nevada, Las VegasFollow
Arthuro Mehretu, University of Nevada, Las Vegas
Mark P. Buttner, University of Nevada, Las VegasFollow
Katherine M. Howard, University of Nevada, Las VegasFollow
Theresa Trice, University of Nevada, Las VegasFollow

Document Type

Article

Publication Date

8-2014

Publication Title

BMC Oral Health

Volume

15

Issue

95

First page number:

1

Last page number:

8

Abstract

Background

In recent studies, periodontal health has been linked to being overweight and/or obese. Among common oral bacteria, Selenomonas noxia has been implicated in converting periodontal health to disease, and Selenomonas species have also been found in gastric ulcers. The objective of this study was to develop and validate a quantitative polymerase chain reaction (qPCR) assay for the specific and rapid detection of S. noxia.

Methods

Two oligonucleotide primer pairs and one probe were designed and tested to determine optimal amplification signal with three strains of S. noxia. The PCR assay was tested against fourteen non-target organisms, including closely related oral Selenomonads, one phylogenetically closely related bacterium, and two commonly isolated oral bacteria.

Results

One of the primer sets was more sensitive at detecting the target organism and was selected for optimization and validation experiments. The designed primers and probe amplified the target organism with 100 % specificity. PCR inhibition was observed with an internal positive control, and inhibition was resolved by diluting the DNA extract.

Conclusions

The qPCR assay designed in this study can be used to specifically detect S. noxiain the clinical setting and in future research involving the enhanced detection of S. noxia. The assay can also be used in epidemiological studies for understanding the role of S. noxia in disease processes including, but not limited to, oral health and obesity of infectious origin.

Keywords

Bacteria; PCR; Periodontal health and disease; Selenomonas noxia

Disciplines

Public Health

Language

English

Publisher Citation

Cruz, P., A.M. Mehretu, M.P. Buttner, K.M. Howard, and T. Trice. Development of a new polymerase chain reaction assay for the rapid detection of the oral pathogenic bacterium, Selenomonas noxia. BMC Oral Health.

Repository Citation

Cruz-Perez, P., Mehretu, A., Buttner, M. P., Howard, K. M., Trice, T. (2014). Development of a New Polymerase Chain Reaction Assay for the Rapid Detection of the Oral Pathogenic Bacterium, Selenomonas Noxia. BMC Oral Health, 15(95), 1-8.
https://digitalscholarship.unlv.edu/env_occ_health_fac_articles/143