Document Type

Article

Publication Date

10-1999

Publication Title

The Auk

Publisher

American Ornithologists' Union

Volume

116

Issue

4

First page number:

1021

Last page number:

1038

Abstract

We examined patterns of variation in skeletal morphometrics (29 characters), allozymes (34 loci), mitochondrial DNA (mtDNA) restriction sites (n = 74) and fragments (n = 395), and mtDNA sequences (1,739 bp from cytochrome b, ND2, ND6, and the control region) among all species of Toxostoma. The phenetic pattern of variation in skeletal morphometrics generally matched traditional taxonomic groupings (based on plumage patterns) with the exceptions of T. redivivum, which because of its large size clusters outside of its proper evolutionary group (lecontei), and T. occelatum, which did not cluster with T. curvirostre. Skull characters contributed highly to species discrimination, suggesting that unique feeding adaptations arose in different species groups. Although genetic variation was detected at isozyme loci (average heterozygosity = 3.6%), these data yielded little phylogenetic resolution. Similarly, mtDNA restriction sites were relatively uninformative; hence, phylogenetic conclusions were based on sequence data. Phylogenetic analyses confirmed the monophyly of these traditionally recognized assemblages: rufum group (T. rufum, T. longirostre, and T. guttatum), lecontei group (T. lecontei, T. crissale, and T. redivivum), and cinereum group (T. bendirei and T. cinereum). The cinereum and lecontei groups appear to be sister lineages. Monophyly of the curvirostre group (which also includes T. occelatum) was not confirmed. Sequence data suggest that T. occelatum and T. curvirostre, which differ by 7.7% sequence divergence, are probably most closely related to the rufum group. Toxostoma rufum and T. longirostre have similar external appearances and differ by 5.0%. Toxostoma guttatum is restricted to Cozumel Island and often is considered a subspecies of T. longirostre; it differs by more than 5% from the other two members of the rufum group and is a distinct species constituting the basal member of this group. The phenotypically distinctive T. bendirei and T. cinereum differ in sequence divergence by only 1.6%. Overall, mtDNA distances computed from coding genes (mean 8.5%) exceeded distances computed from the control region (mean 7.6%), contrary to expectation. Because neither allozymes nor mtDNA could unambiguously resolve the placement of T. occelatum and T. curvirostre, a scenario involving contemporaneous speciation is suggested. Application of a molecular clock suggested that most speciation occurred in the late Pliocene or early Pleistocene.

Keywords

Birds--Speciation; Divergence (Biology); Molecular genetics; Phylogeography; Toxostoma

Disciplines

Ecology and Evolutionary Biology | Genomics | Molecular Genetics | Ornithology

Language

English

Permissions

Published as The Auk Vol. 116, No. 4, Oct., 1999. © 1999 by the Regents of the University of California/The American Ornithologists' Union. Copying and permissions notice: Authorization to copy this content beyond fair use (as specified in Sections 107 and 108 of the U. S. Copyright Law) for internal or personal use, or the internal or personal use of specific clients, is granted by the Regents of the University of California/on behalf of The American Ornithologists' Union for libraries and other users, provided that they are registered with and pay the specified fee via Rightslink® on [JSTOR (http://www.jstor.org/r/ucal)] or directly with the Copyright Clearance Center, http://www.copyright.com

Identifier

DOI: http://ezproxy.library.unlv.edu/login?url=http://dx.doi.org/10.2307/4089682