Determination of Microvessel Permeability and Tissue Diffusion Coefficient of Solutes by Laser Scanning Confocal Microscopy

Authors

Bingmei M. Fu, City University of New YorkFollow
Roger H. Adamson, University of California at DavisFollow
Fitz-Roy E. Curry, University of California at Davis

Document Type

Article

Publication Date

4-2005

Publication Title

Journal of Biomechanical Engineering

Volume

127

Issue

2

First page number:

270

Last page number:

278

Abstract

Interstitium contains a matrix of fibrous molecules that creates considerable resistance to water and solutes in series with the microvessel wall. On the basis of our preliminary studies, by using laser-scanning confocal microscopy and a theoretical model for interstitial transport, we determined both microvessel solute permeability (P) and solute tissue diffusion coefficient (D) of alpha-lactalbumin (Stokes radius 2.01 nm) from the rate of tissue solute accumulation and the radial concentration gradient around individually perfused microvessel in frog mesentery. P(alpha-lactalbumin) is 1.7 +/- 0.7(SD) x 10(-6) cm/s (n = 6). D(t)/D(free) for alpha-lactalbumin is 27% +/- 5% (SD) (n = 6). This value of D(t)/D(free) is comparable to that for small solute sodium fluorescein (Stokes radius 0.45 nm), while p(alpha-lactalbumin) is only 3.4% of p(sodium fluorescein). Our results suggest that frog mesenteric tissue is much less selective to solutes than the microvessel wall.

Keywords

Diffusion; Frogs; Mesentery; Tissues – Permeability

Disciplines

Biomechanics and Biotransport | Biomedical Engineering and Bioengineering | Mechanical Engineering | Molecular, Cellular, and Tissue Engineering

Language

English

Permissions

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Repository Citation

Fu, B. M., Adamson, R. H., Curry, F. E. (2005). Determination of Microvessel Permeability and Tissue Diffusion Coefficient of Solutes by Laser Scanning Confocal Microscopy. Journal of Biomechanical Engineering, 127(2), 270-278.