Title

Structural Mechanisms of Acute VEGF Effect on Microvessel Permeability

Document Type

Article

Abstract

To investigate the ultrastructural mechanisms of acute microvessel hyperpermeability by vascular endothelial growth factor (VEGF), we combined a mathematical model (J Biomech Eng 116: 502–513, 1994) with experimental data of the effect of VEGF on microvessel hydraulic conductivity (L p) and permeability of various-sized solutes. We examined the effect of VEGF on microvessel permeability to a small solute (sodium fluorescein, Stokes radius 0.45 nm), an intermediate solute (α-lactalbumin, Stokes radius 2.01 nm), and a large solute [albumin (BSA), Stokes radius 3.5 nm]. Exposure to 1 nM VEGF transiently increased apparent permeability to 2.3, 3.3, and 6.2 times their baseline values for sodium fluorescein, α-lactalbumin, and BSA, respectively, within 30 s, and all returned to control within 2 min. On the basis ofL p (DO Bates and FE Curry. Am J Physiol Heart Circ Physiol 271: H2520–H2528, 1996) and permeability data, the prediction from the model suggested that the most likely structural changes in the interendothelial cleft induced by VEGF would be a ∼2.5-fold increase in its opening width and partial degradation of the surface glycocalyx.

Disciplines

Biomechanics and Biotransport | Biomedical Engineering and Bioengineering | Molecular, Cellular, and Tissue Engineering

Permissions

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