Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice

Authors

Jennifer Nicholas, University of Nevada, Las VegasFollow
Joachim G. Voss, University of Washington - Seattle CampusFollow
Joyce Tsuji, University of Washington - Seattle Campus
Nadia D. Fulkerson, University of Nevada, Las VegasFollow
Julia Soulakova, University of Nebraska-Lincoln
Barbara St. Pierre Schneider, University of Nevada, Las VegasFollow

Document Type

Article

Publication Date

3-24-2014

Publication Title

Innate Immunity

Volume

20

Issue

4

Abstract

Innate pro-inflammatory processes, such as chemokine signaling and leukocyte infiltration, predominate during the first 48 h after an acute skeletal muscle injury. However, the time course of chemokine expression and its relationship to leukocyte infiltration after acute muscle injury within this early post-injury time period has not been investigated. In this study, 46 anesthetized female C57BL/6NHsd mice underwent a closed crush injury of the gastrocnemius muscle and were euthanized 4, 8, 24 and 48 h post-injury. Microarray analysis found 14 chemokine genes to be up-regulated during this period, 12 of which are involved in macrophage or neutrophil chemotaxis, with up-regulation peaking at either 8 or 48 h. RT-PCR analysis on select chemokines confirmed the microarray activation pattern. Neutrophil infiltration patterns mirrored the time course of neutrophil-related chemokines with Gr-1-, 1A8- and 7/4-positive neutrophils infiltrating the muscle 4 h after injury, decreasing at 48 h. Conversely, gene expression and relative quantification levels of macrophage-related chemokines Ccl2 and Ccl7 peaked at 8 h, preceding the infiltration of CD68- and F4/80-positive macrophages, and protein expression of Ccl2 in the muscle. The up-regulation of other macrophage-related chemokines and their receptors peaked at 48 h post-injury.

Keywords

Chemokines; Inflammation; Macrophages; Mice – Wounds and injuries; Microarray; Muscle recovery; Muscles – Wounds and injuries; Neutrophils

Disciplines

Medicine and Health Sciences | Musculoskeletal System | Nursing

Language

English

Repository Citation

Nicholas, J., Voss, J. G., Tsuji, J., Fulkerson, N. D., Soulakova, J., Schneider, B. S. (2014). Time course of chemokine expression and leukocyte infiltration after acute skeletal muscle injury in mice. Innate Immunity, 20(4),
http://dx.doi.org/10.1177/1753425914527326