Differential gene expression in a murine model of cancer cachexia
Document Type
Article
Publication Date
8-1-2001
Publication Title
American Journal of Physiology - Endocrinology and Metabolism
Volume
281
Issue
2
First page number:
E289
Last page number:
E297
Abstract
Murine adenocarcinoma 16 (MAC16) tumors and cell lines induce cachexia in NMRI nude mice, whereas histologically similar MAC13 tumors do not. After confirming these findings in BALB/c nude mice, we demonstrated that this tissue wasting was not related to decreased food intake or increased total body oxidative metabolism. Previous studies have suggested that MAC16's cachexigenic properties may involve the production of tumor-specific factors. We therefore screened for genes having increased expression in the MAC16 compared with the MAC13 cell line by performing hybridization to a murine cDNA expression array, by generation and comparison of cDNA libraries from each cell line, and by PCR-based subtractive hybridization. Northern blot hybridization was performed to confirm differences in transcript expression. Transcripts encoding insulin-like growth factor binding protein-4, cathepsin B, ferritin light and heavy chain, endogenous long-terminal repeat sequences, and a viral envelope glycoprotein demonstrated increased expression in the MAC16 cell line. The roles of a number of these genes in known metabolic pathways identify them as potential participants in the induction of cachexia.
Keywords
Array; Cachexia; Cancer; Cdna Expression; Gene expression; Genes; Murine Adenocarcinoma 13 and 16 Cells; Tumors
Disciplines
Anesthesiology | Cancer Biology | Critical Care | Genetics and Genomics | Otorhinolaryngologic Diseases | Pathology
Language
English
Repository Citation
Monitto, C. L.,
Berkowitz, D.,
Lee, K. M.,
Pin, S.,
Li, D.,
Breslow, M.,
O’malley, B.,
Schiller, M. R.
(2001).
Differential gene expression in a murine model of cancer cachexia.
American Journal of Physiology - Endocrinology and Metabolism, 281(2),
E289-E297.