Genes expressed in the mouse pituitary corticotrope AtT-20/D-16v tumor cell line

Document Type

Article

Publication Date

11-2000

Publication Title

Pituitary

Volume

3

Issue

3

First page number:

141

Last page number:

152

Abstract

The pituitary corticotrope AtT-20 stable cell line has been used as a model system to study peptide secretion, glucocorticoid regulation, and several other processes. In order to better understand this model cell line, a phage cDNA library was generated from AtT-20/D-16v cell mRNA and cDNA sequences were obtained for 317 clones representing 203 known genes and 48 novel cDNAs. The sequencing results revealed the prevalence of the mouse leukemia virus in this cell line and also identified a number of putatively secreted molecules that were not previously recognized as being secreted from AtT-20/D-16v cells or pituitary corticotropes. Nine completely novel cDNAs and 39 cDNAs homologous to known ESTs were also identified. A listing of other genes known to be expressed in AtT-20/D-16v cells is also provided.

Keywords

Adrenocorticotropic Hormone/secretion; Animals; Cancer; Cell lines; DNA; DNA; Complementary/genetics; Gene expression; Gene libraries; Gene Library; Leukemia; Mice; Pituitary gland; Pituitary Gland; Anterior; Pituitary Neoplasms/genetics; Pituitary Neoplasms/secretion; Tumor Cells; Cultured/physiology; Tumors

Disciplines

Cancer Biology | Cell and Developmental Biology | Cellular and Molecular Physiology | Genetics and Genomics | Neuroscience and Neurobiology

Language

English

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