Document Type
Article
Publication Date
3-31-2021
Publication Title
Nature Communications
Volume
12
Issue
1
First page number:
1986
Abstract
Many bacteria use the second messenger cyclic diguanylate (c-di-GMP) to control motility, biofilm production and virulence. Here, we identify a thermosensory diguanylate cyclase (TdcA) that modulates temperature-dependent motility, biofilm development and virulence in the opportunistic pathogen Pseudomonas aeruginosa. TdcA synthesizes c-di-GMP with catalytic rates that increase more than a hundred-fold over a ten-degree Celsius change. Analyses using protein chimeras indicate that heat-sensing is mediated by a thermosensitive Per-Arnt-SIM (PAS) domain. TdcA homologs are widespread in sequence databases, and a distantly related, heterologously expressed homolog from the Betaproteobacteria order Gallionellales also displayed thermosensitive diguanylate cyclase activity. We propose, therefore, that thermotransduction is a conserved function of c-di-GMP signaling networks, and that thermosensitive catalysis of a second messenger constitutes a mechanism for thermal sensing in bacteria.
Disciplines
Bacteriology
File Format
File Size
2964 KB
Language
English
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.
Repository Citation
Almblad, H.,
Randall, T.,
Liu, F.,
Leblanc, K.,
Groves, R.,
Kittichotirat, W.,
Winsor, G.,
Fournier, N.,
Au, E.,
Groizeleau, J.,
Rich, J.,
Lou, Y.,
Granton, E.,
Jennings, L.,
Singletary, L.,
Winstone, T.,
Good, N.,
Bumgarner, R.,
Hynes, M.,
Singh, M.,
Stietz, M.,
Brinkman, F.,
Kumar, A.,
Brassinga, A.,
Parsek, M.,
Tseng, B.,
Lewis, I.,
Yipp, B.,
MacCallum, J.,
Harrison, J. J.
(2021).
Bacterial Cyclic Diguanylate Signaling Networks Sense Temperature.
Nature Communications, 12(1),
1986.
http://dx.doi.org/10.1038/s41467-021-22176-2