Measuring nitrification, denitrification, and related biomarkers in continental geothermal ecosystems
Document Type
Article
Publication Date
2011
Publication Title
Methods in Enzymology
Volume
486
First page number:
171
Last page number:
203
Abstract
Research on the nitrogen biogeochemical cycle in terrestrial geothermal ecosystems has recently been energized by the discovery of thermophilic ammonia-oxidizing archaea (AOA). This chapter describes methods that have beenused for measuring nitrification and denitrification in hot spring environments,including isotope pool dilution and tracer approaches, and the acetylene blockapproach. The chapter also summarizes qualitative and quantitative methodsfor measurement of functional and phylogenetic biomarkers of thermophilespotentially involved in these processes
Keywords
Ammonia – oxidation; Archaebacteria; Biochemical markers; Denitrification; Hot spring ecology; Nitrification; Nitrifying bacteria; Nitrogen – Fixation; Thermophilic bacteria; Thermophilic microorganisms
Disciplines
Bacteriology | Environmental Microbiology and Microbial Ecology | Life Sciences | Microbiology | Systems Biology
Language
English
Repository Citation
Dodsworth, J. A.,
Hungate, B. A.,
Torre, J. d.,
Jiang, H.,
Hedlund, B. P.
(2011).
Measuring nitrification, denitrification, and related biomarkers in continental geothermal ecosystems.
Methods in Enzymology, 486
171-203.
http://dx.doi.org/10.1016/S0076-6879(11)86008-