Differential Effects of Methylisothiazolinone (MIT) on Dental Pulp Stem Cells (DPSC)

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Journal of Medical Discovery





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Background: Methylisothiazolinone (MIT) is a strong preservative commonly found in household products such as cosmetics, soaps and baby wipes and may be linked with acute contact dermatitis (ACD). Evidence has now emerged that demonstrates the effects of MIT may not be limited to ACD and other skin allergies, but may also affect other tissue types, including stem cells. Relatively few studies have evaluated the effects of this compound on oral cells and tissues, therefore the primary objective of this study was to evaluate the effects of MIT on dental pulp stem cells (DPSC), which may be acutely sensitive to differentiation stimuli and environmental exposures. Methods: Two dental pulp stem cell (DPSC) isolates (dpsc-11750, dpsc-11836) from an existing repository were selected for this study to test the effects of MIT. Cell viability was measured using the Trypan Blue exclusion assay. Cellular proliferation was measured using 96-well growth assays using different concentrations of MIT ranging between 25–75 uM. Results: Differential effects of MIT were observed with DPSC viability. Comparison of baseline (control: 0 uM) viability of DPSC isolates revealed significantly increased viability among dpsc-11836 cells at all concentrations (25 uM, 50 uM, 75 uM, P=0.004) with significantly reduced viability among dpsc-11750 cells at all concentrations (P=0.03). In addition, differential effects of MIT were also observed with DPSC proliferation. Comparison of baseline (control: 0 uM) growth of DPSC isolates revealed significantly increased proliferation among dpsc-11836 cells at all concentrations (25 uM, 50 uM, 75 uM, P=0.05) with significantly reduced growth among dpsc-11750 cells at all concentrations (P=0.04). Conclusions: Because MIT and other biocides are used in a wide variety of consumer products and industrial processes, a greater understanding of the potential effects of these compounds on specific cells and tissue types is warranted. As differential responses to MIT were observed among the DPSC isolates evaluated, a more research will be needed to elucidate the factors that regulate and mediate the cellular responses to MIT exposure.


Methylisothiazolinone; MIT; Dental pulp stem cells; DPSC


Dentistry | Medicine and Health Sciences



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