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Inositol Polyphosphate Multikinase (IPMK) is a relatively new gene found in mammalian cells. To determine where I should further conduct our research, I localized this gene within HEK-293 cells. For this study, HEK-293 cells are used. These cells are easily cultured and transfected, both of which are needed for this study. IPMK tagged with Green Fluorescent Protein is transfected into our test group. The control group is transfected with only Green Fluorescent Protein. After transfecting the HEK-293 cells, the cells are put under a fluorescent microscope under the GFP setting. In the HEK-293 cells that were transfected with IPMK GFP, the visible green fluorescence appeared within the nucleus of the cell. In the HEK-293 cells that were transfected with only GFP, the whole cell was seen with green fluorescence. With this information, I conclude that IPMK is localized within the nucleus of the cell, thus giving the information on where I should begin my research on this gene.
Las Vegas (Nev.)
University of Nevada, Las Vegas
Cell nuclei; Methods in cell biology
Cell and Developmental Biology | Cells
Sio, Braden, "Localization of IPMK" (2022). Undergraduate Research Symposium Posters. 136.
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Faculty Mentor: Prasun Guha