Download Full Text (666 KB)
As a result of the COVID-19 pandemic, it is imperative to develop novel and rapid ways to synthesize highly targeted and effective vaccines. We report a study that tested the effectiveness of using electrochemistry to damage the Tobacco Mosaic Virus (TMV). We hypothesized that by utilizing the acidic properties of DNA and RNA contained by viruses, viral genetic material can be selectively denatured using electrochemistry. We also hypothesized that the viral capsid would be less damaged due to its non-acidic properties and thus the intact capsid could be used as a vaccine vector to promote immune system response. We tested TMV potency by infecting pinto bean seedlings with electrochemically treated and untreated TMV and observed their growth for four weeks. Various spectroscopic techniques such as Nuclear Magnetic Resonance Spectroscopy (NMR) and Ultraviolet-Visible (UV-Vis) Spectroscopy were implemented to assess changes in the electrochemically-treated viruses. Finally, Cyclic Voltammetry (CV) was used to examine oxidative damage in the samples. It was observed that plants infected with electrochemically-treated TMV developed significantly fewer necrotic lesions compared to plants infected with untreated TMV. We also found significant changes in the UV-Vis spectral peaks, NMR spectral peaks, and cyclic voltammograms before and after electrochemical damage. Preliminary results were obtained which suggest that the electrochemical techniques used in this study effectively damaged the TMV virions. Through further testing and improvements, this innovative technique could be used to develop highly targeted and effective vaccines.
Tobacco mosaic virus; Electrochemistry; Vaccine; Cyclic voltammetry
Diaz Tremillo, Angelica; Ayala Pineda, Kevin; Pudar, Nicholas; Joseph, Drake; and Pravica, Michael, "Damaging Tobacco Mosaic Virus Using Electrochemistry: A Novel Method to Synthesize High-Quality Vaccines" (2021). Undergraduate Research Symposium Posters. 80.
IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/