Title

Dual recognition of chromatin and microtubules by INC ENP is important for mitotic progression

Document Type

Article

Publication Date

1-1-2017

Publication Title

Journal of Cell Biology

Volume

216

Issue

4

First page number:

925

Last page number:

941

Abstract

The chromosomal passenger complex (CPC), composed of inner centromere protein (INC ENP), Survivin, Borealin, and the kinase Aurora B, contributes to the activation of the mitotic checkpoint. The regulation of CPC function remains unclear. Here, we reveal that in addition to Survivin and Borealin, the single α-helix (SAH) domain of INC ENP supports CPC localization to chromatin and the mitotic checkpoint. The INC ENP SAH domain also mediates INC ENP's microtubule binding, which is negatively regulated by Cyclin-dependent kinase-mediated phosphorylation of segments flanking the SAH domain. The microtubule-binding capacity of the SAH domain is important for mitotic arrest in conditions of suppressed microtubule dynamics, and the duration of mitotic arrest dictates the probability, but not the timing, of cell death. Although independent targeting of INC ENP to microtubules or the kinetochore/centromere promotes the mitotic checkpoint, it is insufficient for a robust mitotic arrest. Altogether, our results demonstrate that dual recognition of chromatin and microtubules by CPC is important for checkpoint maintenance and determination of cell fate in mitosis. © 2017 Wheelock et al.

Language

english

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