Location
University of Nevada, Las Vegas
Start Date
16-4-2011 10:00 AM
End Date
16-4-2011 11:30 AM
Description
Ipsdienol is an important pheromone component for pine engraver beetle, Ips pini. Ipsdienol is a ten carbon monoterpenoid secondary alcohol and ipsdienone is the corresponding ketone. We are characterizing the activity of recombinant IDOL DH produced in Sf9 (insect) cells. The enzyme has a high stereospecificity: (-) ipsdienol was found to be a substrate while (+)-ipsdienol was neither a substrate nor inhibitor. Closely related monoterpenoids, such as nerol, geraniol, and citral, were neither substrates nor inhibitors. Smaller compounds, such as 2-propanol, also failed to act as an inhibitor or substrate. This indicates the binding site of this enzyme is highly selective. Failure to act as an inhibitor most likely indicates these compounds bind weakly (-)-Ipsdienol, ipsdienone, ipsenol, and ipsenone are substrates. Interestingly, menthone, a cyclic analog of ipsdienol, was found to have substrate activity. Results from gel permeation chromatography shows the active conformation of IDOL DH is a tetramer. Together these results suggest IDOLDH has a highly specific substrate binding site, and is a key component in pheromone biosynthesis.
Keywords
Pine engraver; Pheromones
Disciplines
Biochemistry | Chemistry | Entomology | Forest Sciences | Molecular Biology
Language
English
Included in
Biochemistry Commons, Chemistry Commons, Entomology Commons, Forest Sciences Commons, Molecular Biology Commons
Characterization of Ips pini ipsdienol dehydrogenase (IDOL DH)
University of Nevada, Las Vegas
Ipsdienol is an important pheromone component for pine engraver beetle, Ips pini. Ipsdienol is a ten carbon monoterpenoid secondary alcohol and ipsdienone is the corresponding ketone. We are characterizing the activity of recombinant IDOL DH produced in Sf9 (insect) cells. The enzyme has a high stereospecificity: (-) ipsdienol was found to be a substrate while (+)-ipsdienol was neither a substrate nor inhibitor. Closely related monoterpenoids, such as nerol, geraniol, and citral, were neither substrates nor inhibitors. Smaller compounds, such as 2-propanol, also failed to act as an inhibitor or substrate. This indicates the binding site of this enzyme is highly selective. Failure to act as an inhibitor most likely indicates these compounds bind weakly (-)-Ipsdienol, ipsdienone, ipsenol, and ipsenone are substrates. Interestingly, menthone, a cyclic analog of ipsdienol, was found to have substrate activity. Results from gel permeation chromatography shows the active conformation of IDOL DH is a tetramer. Together these results suggest IDOLDH has a highly specific substrate binding site, and is a key component in pheromone biosynthesis.
Comments
Research supported by NSF EPSCoR UROP