Association of IGF-I and IGF-II with myofiber regeneration in vivo
Document Type
Article
Publication Date
3-1999
Publication Title
Muscle & Nerve
Volume
22
Issue
3
First page number:
347
Last page number:
354
Abstract
This study examined expression of insulinlike growth factor (IGF) in the myofibers and nonmyofibrillar structures of murine soleus muscle following contraction-induced damage. Identifying the cellular sources of this myogenic growth factor could improve muscle rehabilitation strategies. Immunohistochemical analysis of muscle sections indicated that the number of myofibers expressing both IGF-I and IGF-II increased significantly at 4, 7, and 10 days following injury, compared with control. Muscle spindles and vascular tissue expressed only IGF-II, and staining intensity did not change following injury. The number of fibers expressing developmental myosin heavy chain increased significantly at 7 and 10 days postinjury, and these usually coexpressed IGF. No IGF-specific staining of interstitial/inflammatory cells was observed. Therefore, expression of IGF after mechanically induced fiber damage occurs exclusively within regenerating fibers without supplemental delivery of IGF to the tissue by inflammatory cells or changes in constitutive expression of IGF-II in vascular tissue.
Keywords
Damage; IGF; Immunohistochemistry; Inflammation; Mice – Wounds and injuries; Muscles – Wounds and injuries; Necrosis; Somatomedin
Disciplines
Medicine and Health Sciences | Nursing
Language
English
Repository Citation
Keller, H. L.,
Schneider, B. S.,
Eppihimer, L. A.,
Cannon, J. G.
(1999).
Association of IGF-I and IGF-II with myofiber regeneration in vivo.
Muscle & Nerve, 22(3),
347-354.
http://dx.doi.org/10.1002/(SICI)1097-4598(199903)22:3<347::AID-MUS7>3.0.CO;2-6