Title

Interdomain conjugal transfer of DNA from Bacteria to Archaea

Document Type

Article

Abstract

Escherichia coli transforms the methanogenic archaeon Methanococcus maripaludis at frequencies ranging from 0.2 × 10−6 to 2 × 10−6 per recipient cell. Transformation requires cell-to-cell contact, oriT, and tra functions, is insensitive to DNase I, and otherwise displays hallmarks of conjugation.

Disciplines

Bacteriology | Genetics and Genomics | Life Sciences | Microbiology