Interdomain conjugal transfer of DNA from Bacteria to Archaea

Authors

Jeremy A. Dodsworth, University of Nevada, Las VegasFollow
Lei Li, Texas A&M UniversityFollow
Shiping Wei, Texas A&M University
Brian P. Hedlund, University of Nevada, Las VegasFollow
John A. Leigh, University of Washington, Seattle
Paul de Figueiredo, Texas A&M University

Document Type

Article

Publication Date

6-2010

Publication Title

Applied and Environmental Microbiology

Volume

76

Issue

16

First page number:

5644

Last page number:

5647

Abstract

Escherichia coli transforms the methanogenic archaeon Methanococcus maripaludis at frequencies ranging from 0.2 × 10⁻⁶ to 2 × 10⁻⁶ per recipient cell. Transformation requires cell-to-cell contact, oriT, and tra functions, is insensitive to DNase I, and otherwise displays hallmarks of conjugation.

Keywords

Archaebacteria; Bacteria; Conjugation (Biology); Escherichia coli; Methanococcus maripaludis

Disciplines

Bacteriology | Genetics and Genomics | Life Sciences | Microbiology

Language

English

Repository Citation

Dodsworth, J. A., Li, L., Wei, S., Hedlund, B. P., Leigh, J. A., Figueiredo, P. d. (2010). Interdomain conjugal transfer of DNA from Bacteria to Archaea. Applied and Environmental Microbiology, 76(16), 5644-5647.
http://dx.doi.org/10.1128/​AEM.00967-10