Master of Public Health (MPH)
First Committee Member
Patricia Cruz-Perez, Chair
Second Committee Member
Third Committee Member
Graduate Faculty Representative
Number of Pages
In recent studies, periodontal health has been linked to being overweight and/or obese. Among common oral bacteria, Selenomonas noxia has been implicated in converting periodontal health to disease. Selenomonas spp. have also been found in gastric ulcers, and were misdiagnosed as Helicobacter -like organisms, but no further studies were conducted. The increasing clinical and epidemiological importance of S. noxia necessitates the development of a rapid detection method. In this study, a TaqMan 16S rRNA based real-time Polymerase Chain Reaction (PCR) method was developed, optimized and evaluated for the rapid and specific detection of S. noxia . The 16S PCR assay using primers and a fluorescent probe was tested against S. noxia , six organisms closely related to S. noxia , and two commonly isolated oral bacteria. The designed primers and probe were optimized, and amplified the target organism with 100% specificity. In conclusion, the 16S real-time PCR designed in this study can be used to specifically amplify S. noxia . The assay can be used for epidemiological studies in understanding the role of S. noxia in disease processes including, but not limited to, oral health and infectobesity.
Biological sciences; Health and environmental sciences; Mouth — Microbiology; Obesity; Oral bacteria; Periodontal disease; Polymerase chain reaction – Diagnostic use; Primers; Selenomonas noxia
Dental Public Health and Education | Microbiology | Molecular Biology | Oral Biology and Oral Pathology
Mehretu, Arthuro, "A polymerase chain reaction method for the detection of Selenomonas noxia" (2011). UNLV Theses, Dissertations, Professional Papers, and Capstones. 1255.