Award Date

5-1-2024

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Interdisciplinary Programs

First Committee Member

Seungman Park

Second Committee Member

Mohamed Trabia

Third Committee Member

Janet Dufek

Fourth Committee Member

Chandrabali Bhattacharya

Number of Pages

53

Abstract

Aging is a risk factor for myriad diseases and is often associated with the accumulation of senescent cells. Cellular senescence, a process of irreversible cell cycle arrest, is associated with various changes in cellular morphology and function. While senescence will occur naturally in a time-dependent manner, it can be prematurely induced by various extrinsic and intrinsic factors. Most senescence studies model cell behavior in two-dimensional (2D) microenvironments; however, these models are not physiologically relevant. In this study, the effects of naturally occurring reactive oxygen species (ROS) hydrogen peroxide (H2O2) and commonly used chemotherapy drugs, doxorubicin and palbociclib, on cellular senescence are modeled and compared in 2D and 3D replicas of skin tissue using human foreskin fibroblasts (HFF). This study shows that senescence associated cellular changes are not only stressor-dependent, but also environment dependent.

Keywords

3D Environment; Aging; Cancer Drugs; Fibroblasts; Hydrogen Peroxide; Senescence

Disciplines

Biomechanical Engineering | Biomedical | Biomedical Devices and Instrumentation

File Format

pdf

File Size

2200 KB

Degree Grantor

University of Nevada, Las Vegas

Language

English

Rights

IN COPYRIGHT. For more information about this rights statement, please visit http://rightsstatements.org/vocab/InC/1.0/

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