University of Nevada, Las Vegas; Center for Academic Enrichment and Outreach
In this work, we explore the histopathological analysis of lung tissue from rodents collected around Boulder City where actinolite was found. Actinolite is an amphibole asbestos that can cause asbestosrelated diseases such as pulmonary fibrosis and inflammation when it is inhaled1. Our goal is to determine if exposure to actinolite has been sufficient enough to cause disease in mammals. The presence of relatively high levels of actinolite collected around Boulder City suggests that people living in the same area could be experiencing ongoing exposure to asbestos fibers and negative health effects2. Studies of the effects of environmental hazards on wild animals can assess health risks in humans3 and provide early warning for public health interventions. We first collected 10 animal subjects around Boulder City then examined the rodents to determine if the presence of actinolite has caused pulmonary fibrosis. Then, different methods of tissue processing were compared to determine which method yields a good slide. Techniques were then developed in histological preparation of lung tissues. Figure
Actinolite; Asbestos; Lung tissue features in rodents
Histopathological Analysis of Lung Tissue Features in Rodents Exposed in Areas with Naturally Occurring Asbestos.
Available at: https://digitalscholarship.unlv.edu/aanapisi_posters/38